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Automatic Protein Purification Instrument——Introduction to Automatic Protein Purification Affinity Chromatography
Separation and purification of fully automatic protein purification equipment is an important means to study protein structure and function. There are a variety of methods for fully automated protein purification instruments. The following schemes are commonly used: salting out or isoelectric point precipitation method based on different design of protein solubility;
2020
07-29
Fully automatic protein purification instrument - a common technology for fully automatic protein separation and purification
Now, many proteins are being discovered without knowing their functions in advance. These naturally need to be separated by fully automatic protein purification equipment for further research to obtain. The operation of fully automated protein purification instruments has now been greatly simplified and improved. _Protein Purification Equipment_High-throughput Protein Purification Instrument_Automatic Protein Purification Instrument
07-26
Fully automatic protein purification equipment - gel filtration chromatography for fully automatic protein separation and purification methods
Because proteins have the characteristics of large particles and different molecular sizes of different proteins, automatic protein purification instruments can separate according to the different sizes of protein molecules. This separation method includes dialysis, ultrafiltration, centrifugation and gel filtration. Among them, gel filtration is one of the most effective methods for separating protein mixtures based on molecular size, also known as size exclusion chromatography or molecular sieve chromatography.
07-24
Fully automatic protein purification instrument - the significance of buffer replacement in fully automatic protein purification
With the development of molecular biology, more and more researchers have mastered various experimental techniques of molecular biology. Protein purification is very complicated. In addition to ensuring purity, protein products must also maintain their biological activity. The purification process of the fully automatic protein purification instrument must be able to produce the same amount and quality of protein every time, with good repeatability. This requires fully automated protein purification instruments to use a very adaptable method to purify the protein instead of using a method that can obtain pure protein.
07-21
Fully automatic protein purification instrument - what are the methods of fully automatic protein extraction and purification?
The separation and purification of fully automatic protein purification instruments are widely used in biochemical research applications. Fully automatic protein purification equipment should use the inherent similarity and difference between different proteins, use the similarity between various proteins to remove the contamination of non-protein substances, and use the differences of each protein to purify the target protein from other proteins.
Fully automatic protein purification instrument - the treatment method for the loss of protein activity during the automatic protein purification process
During the use of automatic protein purification equipment, when the protein loses its activity, it is necessary to analyze the situation carefully to find out the cause. More importantly, enzyme units should be carefully calculated to assess the extent of loss of viability. As with many automated protein purification instrument steps, the profile of protein activity loss is different for each protein.
07-14
How protein purification equipment maintains protein stability
The protein purification equipment can quickly complete the purification of biomolecular proteins, nucleic acids, peptides, etc., and complete the rapid development and amplification of the entire process from basic laboratory research to the entire process. Protein purification equipment can be used for the purification of biological macromolecules, the purification of known and unknown proteins, the study of protein structure dynamics drug targets, the separation of pharmaceutical proteins, the synthesis of protein engineering drugs, the characterization of proteins, the various How a protein develops and functions in an organism, and the isolation of gene expression products.
07-09
Fully Automated Protein Purification Instruments: General Principles of Protein Purification
With the development of molecular biology, more and more researchers have mastered various experimental techniques of molecular biology, but the upstream work of molecular biology is often not our goal, the key is to obtain pure expression products to study its biological effects. Compared with the upstream work, the protein purification work is very complicated. In addition to ensuring the purity, the protein product must also maintain its biological activity. The purification process of protein purification equipment must be able to produce the same quantity and quality of protein every time, with good repeatability.
07-06
Selection of chromatographic purification methods for automatic protein purification instruments
The automatic protein purification instrument is a compact, convenient and easy-to-use automatic purification equipment. Each component is clearly labeled for quick installation. The unique buffer rack design is convenient for users to place solutions. The number of the instrument corresponds to the operation chart and the label of the prepackaged reagents one by one, which makes it very convenient for users to use. The fully automatic protein purification instrument is operated in touch screen mode, and the switching between interfaces is fast, intuitive and familiar with the help function, so that users can conduct experiments, system cleaning and maintenance through a variety of preset methods.
07-02
Working principle and precautions of protein purification equipment
Protein purification equipment is widely used in biochemical research applications and is an important operating technology. A typical eukaryotic cell contains thousands of different proteins, some abundant and some containing only a few copies. To study a protein, it must first be purified from other proteins and non-protein molecules.
06-30