Now, many proteins are being discovered without knowing their functions in advance. These naturally need to be separated by fully automatic protein purification equipment for further research to obtain. The operation of fully automated protein purification instruments has now been greatly simplified and improved. _Protein Purification Equipment_High-throughput Protein Purification Instrument_Automatic Protein Purification Instrument
Each protein has its own physicochemical properties determined by the sequence of amino acids. It is these differences in physical properties between proteins that allow them to be purified by fully automated protein purification equipment, but this also means that a new set of methods needs to be developed for each protein to be purified. Fortunately, despite this inherent difficulty, a variety of methods are available and protein purification strategies are practical. _Protein Purification Equipment_High-throughput Protein Purification Instrument_Automatic Protein Purification Instrument
Common techniques for automatic protein separation and purification:
2. Electrophoresis: The protein is charged in a solution higher or lower than its isoelectric point, and can move to the positive or negative pole of the electric field in the electric field. Depending on the support, there are thin film electrophoresis, gel electrophoresis, etc.
3. Dialysis: The automatic protein purification instrument uses a dialysis bag to separate macromolecular proteins from small molecular compounds.
4. Chromatography: The fully automatic protein purification instrument utilizes different distribution ratios of proteins between the stationary phase and the mobile phase to achieve the purpose of separation.
a. Ion-exchange chromatography, using the free nature of proteins, at a certain pH, the charge and properties of each protein are different, so it can be separated by ion-exchange chromatography with automatic protein purification equipment. For example, in anion exchange chromatography, proteins with a small negative charge are eluted first.
b. Molecular sieve, also known as gel filtration, small molecular proteins enter the pores and stay for a long time, while large molecular proteins cannot enter the pores at the same time and flow out directly.
5. Ultracentrifugation: It can be used not only to separate and purify proteins but also to determine the molecular weight of proteins. Different proteins have different densities and shapes and are separated.