Automatic Protein Purification Instrument——Introduction to Automatic Protein Purification Affinity Chromatography

Separation and purification of fully automatic protein purification equipment is an important means to study protein structure and function. There are a variety of methods for fully automated protein purification instruments. The following schemes are commonly used: salting out or isoelectric point precipitation method based on different design of protein solubility; dialysis and ultrafiltration or gel filtration method based on protein molecular weight difference; isoelectric focusing based on different design of protein charge Electrophoresis or ion exchange chromatography; and affinity chromatography using specific ligands to bind to the target protein, etc. The characteristics of different proteins vary greatly, and it is often a difficult and complicated task to explore the purification conditions. _Imported protein purification instrument_protein purification equipment_protein purification system_automatic protein purification equipment

There are many methods to capture and purify target proteins from crude extracts or other complex mixtures. The more effective of these methods is affinity chromatography, which achieves the purpose of purification through the specific binding of the target protein to the matching immobilized ligand. _Imported protein purification instrument_protein purification equipment_protein purification system_automatic protein purification equipment

Automatic protein purification instrument Affinity chromatography is a chromatography method designed by using the affinity adsorption and dissociation between biomolecules. It has the characteristics of simple operation, mild conditions, and high purity of the obtained protein. It is especially suitable for the purification of active proteins, and It also has a good separation effect on active proteins with low expression. Commonly used affinity chromatography matrices include: protein A or protein G affinity matrices for antibodies, heparin-based affinity matrices for growth factors and nucleic acid binding proteins, affinity matrices for purification of tagged fusion proteins, and binding Affinity matrices for specific antibodies, etc. _Imported protein purification instrument_protein purification equipment_protein purification system_automatic protein purification equipment

With the wide application of automatic protein purification instrument technology, it has become a very common technique in laboratories to express the target protein in fusion with an affinity purification tag, and then purify the target protein by affinity chromatography. _Imported protein purification instrument_protein purification equipment_protein purification system_automatic protein purification equipment