A secondary antibody and enzyme separation

Instrumnt recommendatione

SDL series

SCG-P series

Filler

UniMab® EXE

Column

6.6×25 mm

Sample

A secondary antibody and enzyme separation

Process steps

Buffer

pH

Volume (CV)

Balance

20 mM Tris-HCl，150 mM NaCl

7.2

4

Sample loading

30 mg/ml

N\A

N\A

Rinse

20 mM Tris-HCl，150 mM NaCl

7.2

3.5

Elution

50 mM NaAC-HAC

3.5

5

Regeneration

1 M HAC

N\A

3

Balance

20 mM Tris-HCl，150 mM NaCl

7.2

2

CIP

0.1M NaOH

N\A

5

Balance

20 mM Tris-HCl，150 mM NaCl

7.2

5

SEC(%)

HCP(ppm)

ProA(ppm)

Yield (%)

98.73

1350

22

96