Double antibody separation reproducibility experiment

Instrument recommendation

SDA series

SCG-P series

Equipment / Materials / Parameters

Describe

Remark

Column

NmVALID 0.8×40 cm

Column height range: 15~25cm
As: 0.8~1.5
HETP: ≥1000N/m

Keep time

5min

N/A

DBC judgment standard

DBC10%

N/A

Sample

Cell clarification

N/A

Filler

UniMab EXE

Batch number: M05N12

Step

Buffer

CVs

Balance

20mM PB+150mM NaCl，pH 7.4

5

Sample loading

Sample

N/A

Wash 1

20mM PB+150mM NaCl，pH 7.4

3

Elution

50mM NaAc-HAc，pH 3.3

Hold

Post disinfection

0.1M NaOH

3

Rinse 2

Purified water

≥3

Store

0.1M sodium acetate + 2% benzyl alcohol, pH 5.0

≥3