Monoclonal antibody isolation reproducibility experiment

Instrument recommendation

SDA series

SCG-P series

Equipment / Materials / Parameters

Describe

Remark

Column

16*300mm

Column height: 15±2cm; As: 0.7~1.8; HETP: ≥1000N/m

Keep time

6min

N/A

DBC judgment standard

DBC10%

N/A

Sample

Cell clarification

N/A

Solutions

Parameter

Remark

Affinity chromatography equilibrium

20mM PB+0.1M NaCl

pH：6.90-7.40conc.:10.00-14.00mS/cm

Affinity chromatography washing solution I

0.1M Sodium citrate+0.5M NaCl

pH：5.80-6.20conc.:55.0-65.0mS/cm

Affinity chromatography eluent

10mM NaAc-HAc

pH：3.20-3.40conc.:0.20-0.30mS/cm

Affinity chromatography disinfectant

0.1M NaOH

N/A

Affinity chromatography preservation solution

N/A

pH：5.00-5.40conc.:N/A