APPLICATION
Monoclonal antibody sample separation and purification
2023-02-08
Instrument recommendation |
SDA series |
SCG-P series |
Sample: |
001 fermentation broth, Titer: 4.01 g/L |
|
Column: |
UniMab EXE (column height: 10.0 cm, column volume: 4.65 ml) |
Step |
Route 1 |
Retention time (min) |
Volume (CV) |
CIP |
0.1 mol/L NaOH |
5 |
3 |
Balance |
20 mmol/L PB, 150 mmol/L NaCl, pH 7.2 |
5 |
3 |
Sample loading |
Loading capacity 30 g/L |
5 |
N/A |
Balance |
20 mmol/L PB, 150 mmol/L NaCl, pH 7.2 |
5 |
5 |
Rinse 1 |
20 mmol/L PB, 1mol/L NaCl pH 7.2 |
5 |
5 |
Rinse 2 |
20 mmol/L PB, pH 7.2 |
5 |
3 |
Elution |
20 mmol/L HAC-NaAC , pH 3.5 |
5 |
100~100 mAU/2 mm |
CIP |
1 mmol/L HAC |
5 |
5 |
Balance |
20 mmol/L PB, 150 mmol/L NaCl, pH 7.2 |
5 |
3 |
Save |
20% ethanol |
5 |
2 |