Modified protein separation experiment

Instrument recommendation

SDA series

SCG-P series

Column:

NmLOAD 50/600,NW Super 75

Mobile phase:

50mM PB+0.1M NaCl （pH 6.8）

Configuration:

6.12g sodium dihydrogen phosphate + 6.95g disodium hydrogen phosphate + 11.68g sodium chloride, add water to 2L, ultrasonically dissolve and filter.

Flow rate:

1.5ml/min

Detection wavelength:

254nm/214nm/280nm

Loading conditions:

Quantitative cup loading, 10mg/ml injection 10ml, each loading 100mg.