Ion exchange packing

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Chromatography column and packing

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Description

Agarosix FF ion exchange packing

Agarosix Fast Llow (Agarosix FF) is specially designed for ion exchange chromatography. The matrix is composed of agarose gel with a particle size of 50-150μm (average 90μm) and a crosslinking degree of 6%. The high loading and high stability shown in the separation are not affected by the mobile phases and cleaning methods commonly used in preparative purification chromatography. The high hydrophilicity of the packing surface reduces the non-specific adsorption between the sample and the stationary phase. The proprietary chemical modification technology ensures the high density and uniformity of the surface ion exchange layer. Agarosix FF-SP, Q, CM, DEAE packing can be widely used in the separation and purification of biological samples.

Technical Parameters:

* Agarosix FF-SP / CM dynamic exchange load determination method: flow rate is 75 cm / h, 0.1 M acetate (pH 5.0) as mobile phase, sample is ribonuclease A (1.0 mg / mL), load Based on 50% flow-through. Agarosix FF-Q / DEAE: flow rate 75 cm / h, 50 mMTris (pH 7.5), 2.0 mg / mL human serum albumin, 50% flow-through meter.

Packing characteristics:

• High ion exchange capacity and high sample load

• 6% crosslinking degree can be used for high flow separation and purification

• High resolution, high efficiency and high recovery rate

• High batch-to-batch reproducibility

• Easy to zoom in

• Surface is highly hydrophilic

Packaging Information:

filler	Particle size	Packing specification (L)	Specification of prepacked column (mL)
Agarosix FF-SP	90 µm	0.1, 0.5, 1, 5, 10	1, 5
Agarosix FF-CM	90 µm	0.1, 0.5, 1, 5, 10	1, 5
Agarosix FF-Q	90 µm	0.1, 0.5, 1, 5, 10	1, 5
Agarosix FF-DEAE	90 µm	0.1, 0.5, 1, 5, 10	1, 5