PRODUCTS
Description
The SCG protein chromatography system is specially designed for the purification and preparation of biological samples such as proteins, antibodies, and vaccines. The modular, functional and intelligent design is easy to operate, and it is convenient for customers to choose a purification system that is more suitable for them according to their actual needs.
SCG protein chromatography system can be used for:
Protein purification; vaccine purification; monoclonal antibody purification; blood product separation and purification.
Peptide purification; gene therapy medicine purification; natural medicine and polysaccharide purification fields.
System Features:
Reliable system components
The main components are manufactured by selected well-known European and American manufacturers. Most of them are developed in cooperation with superior performance and reliable quality.
All components in contact with the sample are made of bio-inert materials such as PEEK, sapphire, and ruby, and have good biocompatibility.
Accurate and continuous liquid flow rate
The original imported dual plunger rod binary gradient pump, the pump head is made of PEEK material, and the front design is easy to clean and maintain.
The pump head has a self-flushing function to avoid salt precipitation in the pump head when purifying biological samples, causing instrument damage and pollution; the infusion pump uses electronic pressure pulse suppression technology to provide excellent gradient accuracy and repeatability for protein chromatography To ensure the reproducibility of purification results.
Accurate and real-time detection environment
The UV detector is an original imported DAD detector, which provides multiple wavelength signal output at the same time, which can facilitate the real-time monitoring of the purity of the separated components; the pH / conductivity detector can accurately provide real-time monitoring of pH and conductivity, and Temperature compensation of pH and conductivity is needed to obtain more accurate monitoring.
Intelligent configuration, enjoy the fun of work
The new component collector is equipped with a variety of collection racks and supports a variety of collection methods to facilitate the purification and collection of the target; various valves (collection valve, column position valve, injection valve, column selection valve, solenoid valve, etc.) are Customized for international well-known manufacturers, imported from the original, users can choose according to actual needs.
Professional and timely after-sales service team
The professional after-sales service team will reply within 2 hours and arrive at the site to solve the problem within 24 hours.
Free software upgrades for life.
Humanized software design:
All SCG systems from laboratory scale to production scale can be controlled using the SCG software developed by our company, which has built-in method editing, system control, result processing and many other information. The main features of SCG software are: intuitive software design, powerful spectrum processing capabilities, safe data storage function, and built-in parameters of chromatographic columns commonly used by domestic and foreign manufacturers.
The Chinese and English operation interfaces are interchangeable, the operation is simple, the function is comprehensive, and it is easy to learn and use. The data is automatically saved in real time to prevent data loss caused by accidental power failure; SCG's unique mobile phone tablet remote control function allows you to monitor the progress of the experiment at any time in the office area; meets GMP / GLP specifications and meets FDA and CFDA regulations .
Protein purification system SCG-030 series
● Adopting high-precision plunger pump, the flow rate and gradient changes are more accurate, and the gradient and flow rate can be modified online in real time.
● Equipped with highly sensitive detectors, and can be equipped with different detectors such as different UV, pH, conductivity, etc.
● Fully automatic component collector, supporting multiple collection methods.
Basic configuration and parameters:
model |
SCG-030 |
System pump |
Plunger pump, bio-inert PEEK material, can provide continuous, stable and accurate flow rate |
Velocity range |
0.001 ~ 36 mL / min |
Maximum pressure |
270bar (27MPa or 4000psi) * |
mixer |
Dynamic, 2mL |
Flow rate accuracy |
± 1.2% |
Gradient type |
Isocratic, linear gradient, step gradient, the gradient ratio can be modified online |
UV detector |
DAD 400EX, slope length 200-400nm (other detectors can be optional) |
light source |
Imported deuterium lamp |
Wavelength accuracy |
± 1nm |
Conductivity detector |
0.001-999.99mS / cm, accuracy ± 2% |
pH detector |
Detection range pH 0-14, accuracy ± 0.1 |
Temperature detector |
Detection range 0-100 ℃, accuracy ± 1 ℃ |
Injection valve |
Automatic sample injection, software control switch Load, Inject, Waste function, support quantitative loop and quantitative cup injection |
Chromatography workstation |
SCG chromatography workstation (including workstation software, brand computer, genuine Win7 operating system) |
Installation kit |
PEEK / PTFE pipe, installation manual, user manual, pipe joint, common tools, etc. |
power supply |
220VAC/110VAC |
Operating temperature |
4-40 ℃ |
Collection device |
SCG FC-S2, supports collection brackets of different specifications |
Collection method |
Manual, full collection, peak collection |
Appearance size (mm) |
System pump: 625 * 485 * 376 Detector: 620 * 510 * 320 Collector: 400 * 400 * 340 |
* Remarks: 1Mpa = 10bar = 145psi
SCG series optional list:
UV detector * |
DF1 single wavelength detector |
280nm, can only monitor 1 wavelength, 2mm optical path |
DF2EX UV detector |
280 & 260nm, simultaneously monitor 2 wavelengths, 2mm optical path |
|
DAD 600EX UV detector |
200-600nm, simultaneously monitor 4 wavelengths, 2mm optical path |
|
DAD 800EX UV detector |
190-840nm, monitor 4 wavelengths at the same time, 2mm optical path |
|
Differential detector |
RI050 differential detector |
2.5-5120uRIU (flow rate range: 1-50ml / min) |
Sample pump |
Pump type |
High precision plunger rod pump, good biocompatibility |
parameter |
0.001 ~ 36ml / min (4000psi ; 0.01 100 ~ 100ml / min (1500psi) |
|
Entrance |
Standard: 1 entrance |
|
Optional: 10 inlets (sample selection valve) |
||
Dynamic mixer |
Mixed mode |
Magnetic stirring |
Mixing chamber volume |
SCG-030: Standard 2ml, optional 0.6ml or 5ml |
|
SCG-100: Standard 2ml, optional 5ml or 10ml |
||
SCG-300: Standard configuration 5ml, optional 10ml or 15ml |
||
System pump inlet |
A pump inlet |
SCG030: 1 standard, 2 or 8 optional |
SCG100: 1 standard, 2 or 8 optional |
||
SCG300: 1 standard, 8 optional |
||
B pump inlet |
SCG030: 1 standard, 2 or 8 optional |
|
SCG100: 1 standard, 2 or 8 optional |
||
SCG300: 1 standard, 8 optional |
||
Column position matching |
Column valve |
Support Column, Bypass and Chromatographic column reverse flow path switching |
Column selection module |
Support to connect 5 chromatographic columns, software control automatic switching |
|
Column differential pressure module |
Support to detect the change of pressure before and after the column, calculate the pressure difference, and protect the packing |
|
Collection module |
Collection valve |
Double-channel large-volume collection valve (1 waste liquid, 1 collection outlet) |
Ten-channel large-volume collection valve (1 waste liquid, 9 collection outlets) |
||
Collector model |
SCG FC-S2, compact in size, can hold 2 collection racks of the same or different specifications |
|
Collection rack type |
16mm collection rack, can hold 60 15ml centrifuge tubes |
|
28mm collection rack for 21 50ml centrifuge tubes |
||
13mm collection rack for 90 5ml centrifuge tubes |
||
Deep well collection rack, can hold 4 96 well plates |
||
Bubble sensor |
When bubbles are detected, the system will pause or enter the next method |
|
Column bracket |
Independent column holder, suitable for ID: 6.6-50mm inner diameter chromatography column and 1ml, 5ml pre-packed column |
|
Wifi module |
Wireless monitoring, suitable for Android phone or tablet |
* The optical path of the UV flow cell is standard equipped with 2mm, and optional 5mm or 10mm.
Flowchart of laboratory protein purification system
Sample pump injection
The flow rate of each pump is 36mL / min, 100mL / min, 300mL / min.
Configuration: dual pumps A1 / A2, B1 / B2, optional 10-port buffer selection valve; including automatic injection pump, and the function of flushing the column in forward and reverse flow; collector, pH, conductivity, ultraviolet, Temperature detection module. The module and pipeline through which the mobile phase passes will be marked in green. In addition, the current state of the module, for example: inject / Load.
Manual injection
The flow rate of each pump is 36mL / min, 100mL / min, 300mL / min.
Double pump A1 / A2, B1 / B2, collector, pH, conductivity, UV, temperature detection module. The modules and lines through which the mobile phase passes are marked in green. Manual injection is possible. After injection, the injection valve will automatically switch to Inject mode according to the set procedure to achieve injection.
System pump injection
The flow rate of each pump is 36mL / min, 100mL / min, 300mL / min.
Double pump A1 / A2, B1 / B2, collector, pH, conductivity, UV, temperature detection module. The modules and lines through which the mobile phase passes are marked in green. When the sample volume is relatively large, the system pump can be used for sample injection, and the sample can be directly input into the chromatography column through the system pump.