For the electrophoresis of protein purification methods, the editor of the SCG protein purification system said his own opinion, and hope to have some reference for you.
Acrylamide gel electrophoresis is commonly used to view the complexity of protein mixture samples and monitor the effectiveness of purification. The separation effect of this method is excellent, but unfortunately it is difficult to scale up to the preparation scale without losing accuracy, because as the thickness of the gel increases, the thermal effect during electrophoresis can seriously interfere with the swimming of the protein.
In basic research, sometimes only a small amount of pure protein is required for research, such as protein sequencing, etc. At this time, electrophoretic purification is a simple and fast method. Acrylamide gel electrophoresis is also an important analytical tool in the protein purification process. It can detect the gradient of the ion exchange column salt eluent of the target protein; it can be used to determine the protein purification technology with the rapid development of various disciplines in recent years. The demand for the product is increasing, the existing purification methods are being improved day by day, and new types of purification methods are emerging one after another. Hydroxyapatite is a crystal of calcium phosphate. Due to its insufficient physical and chemical properties and poor binding ability, it is difficult to use for chromatography.
Recently, the company has improved it to increase the ratio of calcium and phosphorus, so that spherical, porous, and stable ceramic hydroxyapatite particles can be formed. The positively charged calcium ions and negatively charged phosphate ions can be The carboxyl and amino groups of the protein are combined. By adjusting the pH of the buffer, acidic and basic amino acids can be selectively combined with this resin, and the salt concentration of the buffer can be changed to elute and separate the protein. The data shows that using this method can make two proteins with the same isoelectric point, molecular weight and hydrophobicity well separated.
The above is about the electrophoresis of protein purification method. If you have the need to order SCG protein purification system, please contact us Suzhou Sepure Instrument Co., Ltd .: 18151128155.