SCG protein purification system company introduces the working principle of UV detector
Hello everyone, the following SCG protein purification system company and you talk about the working principle of the UV detector as follows, I hope to help you. The working principle of the ultraviolet detector is based on the law of light absorption. The light emitted from the light source passes through the slit, filter, sample cell to the photoelectric growth tube, so that the change of the light intensity caused by the different sample concentration is converted into the change of the photocurrent. This photocurrent is input to the logarithm through the amplifier The converter converts the transmittance T to the output of A, that is, A = 1g — = εCL where ε is the molar extinction system of the sample to be tested, C is the sample concentration, and the unit is mol / L, and L is the optical path, Use centimeters as the unit. According to the above formula, A is measured and the sample concentration C is known. If the recorder is directly input from the amplifier, the change in the transmittance T of the sample is drawn, and if the input from the logarithmic converter is input to the recorder, the change in the optical density of the sample is drawn.
SCG protein purification system company-Suzhou Sepure Instrument Co., Ltd. is committed to the development of precision purification systems for the separation and purification of proteins, automatic protein chromatography systems, antibodies, natural products and small molecule pharmaceuticals and other biological products. The company has now launched A variety of purification systems with different configurations, from laboratory R & D level to industrial production amplification level, provide customers with a one-stop shopping experience, and strive to provide customers with stable and practical products and a comprehensive product service system. The company has established good cooperation with many well-known biopharmaceutical companies (including CRO companies) at home and abroad, and the product indicators have reached the international leading level of the same type of products, and has received recognition and praise from many customers. SCG Protein Purification System Co., Ltd. welcomes everyone to consult.