NEWS
Domestic FPLC System Company Talking about Normal Phase Chromatography
2020-03-05
The domestic FPLC system company tells you that the groups covalently bound to the carrier in normal phase chromatography are polar groups, such as primary amino, cyano, glycol, dimethylamino and diamino. Mobile phase solvents are non-polar solvents that are very similar to mobile phases in adsorption chromatography, such as heptane, hexane, and isooctane. Since the stationary phase is polar, the stronger the polarity of the mobile solvent, the stronger the elution ability, that is, the solvent with high polarity is a strong solvent. This relationship between the stationary phase and the mobile phase is exactly the same as liquid-solid chromatography, which is called normal phase chromatography.Nonetheless, the separation principle of normal phase chromatography is mainly based on the partition coefficient of the compound in the stationary phase and mobile phase. It is not suitable for separating geometric isomers
Domestic FPLC system company-Suzhou Sepure Instrument Co., Ltd. is committed to developing precision purification systems for biological products such as protein purification, automatic protein chromatography systems, antibodies, natural products and small molecule drugs. A variety of purification systems with different configurations, from laboratory research level to industrial production amplification level, provide customers with a one-stop shopping experience, and strive to provide customers with stable and practical products and a comprehensive product service system. The company has established good cooperation with many well-known biopharmaceutical companies (including CRO companies) at home and abroad, and the product indicators have reached the international leading level of the same type of products, and has received recognition and praise from many customers. Domestic FPLC system companies welcome everyone to come to consult.