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How does protein purification equipment have stability in protein purification?
2020-08-03
Protein purification technology equipment Protein purification can quickly complete the purification of biological intellectual proteins, nucleic acids, peptides, etc., and can realize rapid improvement and development from laboratory safety basic education research to the entire production process analysis method. Protein purification equipment can purify the examples of biological macromolecules, the purification of known and unknown proteins, the study of protein structure system dynamics drug therapy, the separation of drug proteins, the synthesis of protein engineering drugs, the characterization of proteins, gene expression products Separation etc.
Different methods of protein purification using protein purification equipment manipulate proteins extracted from cells with changes in the environment and loss of their activity. Protein purification operations include dilution to change solution conditions, exposure to different enzymatic degradation, heavy metals and oxygen surfaces of various materials, changes in physiological conditions, etc. Note that with any protein purification equipment such conditions may affect protein stability, so taking precautions to minimize these effects as much as possible will help ensure the success of the purification protocol.
Protein Purification Technology Equipment During the process of protein purification, we want to develop and maintain protein stability, and we need to avoid treatment methods that companies can use to denature them. Therefore, protein purification equipment should not vigorously stir or vortex the protein solution, because this may directly cause some protein oxidation or surface denaturation. Protein purification equipment should not expose protein solutions to extremes of pH, high temperature, organic solvents, or any conditions that may promote denaturation. So if long-term storage in the thawed state is also an important protein solution, the growth environment of bacteria and fungi will gradually become a major problem. In such circumstances, sterile solutions and antibacterial or antifungal agents are required. Also, it is recommended to choose to use distilled water for all solutions that will come into contact with the protein, and to store the distilled water in algae-free growth containers according to protein purification equipment management.
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