The characteristics of protein purification of imported protein purifiers can be divided into two different stages. The method used in the normal protein purification system is the resin method. In the stage of rough separation and analysis, the relevant proteins are separated from the main components of other tissue cells such as DNA and RNA. At this time, the sample data of the enterprise is large in size and complex in composition, requiring the resin we use to have high capacity, high flow rate, large particles, and wide particle size distribution. The protein can be quickly separated from the pollutants, and some corresponding protective agents can be added to prevent the binding protein from being degraded. The fine purification stage requires a higher resolution. This stage is to distinguish proteins from those proteins with similar molecular weight and physical and chemical properties. Smaller resin particles are used to improve spatial resolution. Ion information exchange is commonly used. For columns and hydrophobic columns, two important factors, selectivity and column efficiency of the resin to be used, should be considered comprehensively during application.
The characteristics of protein purification of imported protein purifiers, the content of protein purification is very rich, different proteins, different requirements, but in general, the basic principles of protein purification are the same, protein purification utilizes the inherent similarities and differences between different proteins To remove contamination from non-protein substances and to purify the target protein from other proteins. Each protein varies in size, shape, charge, hydrophobicity, solubility, and biological activity, and these differences can be used to extract proteins from mixtures, such as E. coli lysates, to obtain recombinant proteins.
The characteristics of the protein purification of the imported protein purifier, basically the purified protein separation stage is divided into two stages: coarse and fine purification stage. The general protein purification method uses resin. The crude protein in the primary separation stage is separated from other cellular components such as DNA, RNA, etc. Because of the large sample volume at this time, the resin for heterogeneous components with high capacity, high flow rate, large particles, and broad particle size distribution are required. And quickly separate protein contaminants.