What is the principle of protein purification equipment for protein purification?

Protein purification equipment Regarding protein purification, there are many types of protein extraction and preparation products, and there are great differences in properties. They are proteins of the same type. Due to the selection of building materials, the methods are not very different, and they are in different development systems. However, the basic information of the key components in most separations is still common. Most of them are proteins that are soluble in water, dilute salt, dilute acid or dilute alkali solution, and a small number of proteins combined with lipids are soluble in ethanol, butyric acid, etc. in organic solvents such as alcohols. Proteins and enzymes can be extracted, isolated and purified using a variety of different solvents. The difference in the solubility of proteins and enzymes in different solvents mainly depends on the ratio of non-polar hydrophobic groups to polar hydrophilic groups in protein molecules, and secondly depends on the arrangement and dipole moment of these functional groups.

Protein purification equipment Common methods for protein purification separation and purification are:

1. Precipitation

2. Electrophoresis: The protein is charged in a solution above or below its isoelectric point, and can move to the positive or negative pole of the electric field in the electric field. According to the support, there are thin film electrophoresis, gel electrophoresis, etc.

3. Protein purification equipment Regarding protein purification, dialysis: a method of separating small molecule compounds from protein macromolecules using dialysis bags.

4. Chromatography: Ion exchange chromatography, using the free nature of these proteins, at a specific pH of a certain company, the charge and properties of each protein are different, so it can be realized by studying ion information exchange chromatography technology at the same time separate. For example, in anion exchange chromatography, proteins with a small negative charge are eluted first. Molecular sieves, also known as gel filtration. Small proteins are inserted into the pores and have a long residence time, while larger proteins cannot flow in and out of the pores and straight.

5. Protein purification equipment Regarding protein purification, ultracentrifugation: it can be used to achieve separation and purification of protein, or it can be directly used to determine the molecular weight of protein. Proteins of different influences are separated by differences in density and morphological structure.