What is the role of protein purification system
The protein purification system is a flexible and intuitive chromatography system for rapid purification of target products such as proteins, peptides and nucleic acids from micrograms to grams. System data support and various chromatographic techniques need to provide a higher purity automatic control requirement. Differential solubility of proteins using various conventional protein separation methods Solubility protein purification system. Many external factors affect proteins, the same in different solubility, and different protein-specific environmental conditions. Appropriately changing the external conditions, the solubility of the protein mixture controls the components.
Protein Purification Systems pH Control and Isoelectric Precipitation Proteins are generally less soluble at their isoelectric point. Organic solvent separation method The solubility of proteins in different solvents varies greatly, from alkaline insoluble to soluble. The concentration of organic solvents that cause protein precipitation is different, and the separation of proteins can be achieved by controlling the concentration of organic solvents.
Protein purification system temperature, different proteins have different solubility and activity in China at different temperature data. Most of the research proteins are relatively stable at low temperatures, so the separation technology can generally be operated at 0°C or lower.
Protein purification system, from the purification of laboratory research to complete the whole basic process of rapid development and amplification. Examples of biological macromolecules can be performed by purifying known and unknown structures Purified proteins, Protein drug targeting Kinetic studies, Isolated protein drugs, Synthetic drugs Protein engineering, Proteins, How various proteins are developed in tissues and cells For biological function, gene expression products are isolated. Can be detected, separated and purified through a variety of biological samples.
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