Protein purification equipment About the operation steps of protein purification system

Regarding the protein purification system, the separation and purification of protein is widely used in the main application of biochemical research, and it is a very important operating system technology. A typical eukaryotic cell may contain thousands of different proteins, some very informative and some containing only a few. To develop and study a certain protein, we first need to purify the protein from other proteins and non-protein molecules.

Operation steps of protein purification system:

1. Turn on the computer, and then turn on the device.

2. Start the Kylin program and enter the control program.

3. Set the working pressure of the management system to 10Mpa, the flow rate to 5mL/min, and click RUN.

4. Use clean water and then equilibrate with the buffer of the whole system.

5. Clean the sample loop 2-3 times until the A280 absorbance value stabilizes.

6. Columns required for flow and pressure, install columns and balance.

7. Sample topping, elution, collection.

8. Fully wash the column with eluent, then rinse the entire management system and sample loop with water, and then fill the entire system with 20% ethanol.

Protein purification equipment Precautions for the use of protein purification systems:

1. Before use, make sure you have read the instructions on how to use it.

2. Replace one-fifth of ethanol at least half a month.

3. Before use, please check the circulation line in the back cavity of the pump to ensure that it is filled with 20% ethanol. If there is air, suck it out.

4. If the pressure is alarming, first confirm the sample after high-speed centrifugation or filtration, and then check whether the column is intact, if the pressure is still alarming, replace the line filter

5. When the pH electrode is not used, its development must not be placed in the enterprise's current asset pool, nor should it be preserved in the water system. The correct storage and management method is: remove the pH electrode from the flow cell, wash it with distilled water first, wipe the water lightly, and store it in a saturator.