Protein Purification Equipmen Regarding Protein Purification There are many types of protein extraction and preparation products, their properties are very different, they are similar proteins. The methods are not very different due to the choice of building materials, and they are in different systems. But basic information about most of the key components of the separation effort is still prevalent. Most proteins are soluble in water, dilute salt, dilute acid or dilute alkali solution, and a few proteins combined with lipids are soluble in ethanol and butanol. in organic solvents such as ethanol. Various solvents are available for extraction, separation and purification of proteins and enzymes. The difference in solubility of proteins and enzymes in different solvents depends primarily on the ratio of non-polar hydrophobic groups to polar hydrophilic groups in the protein molecule, and secondarily on the arrangement and dipole moment of these functional groups.
Common methods for protein purification equipment for protein separation and purification are:
1. Precipitation and electrophoresis: The protein is charged in a solution above or below its isoelectric point, and can move to the positive or negative electrode of the electric field in the electric field. According to the support, there are thin film electrophoresis, gel electrophoresis, etc.
2. Protein purification equipment Regarding protein purification and dialysis: a method of separating small molecular compounds from protein macromolecules using dialysis bags.
3. Chromatography: Ion-exchange chromatography, using the free characteristics of these proteins, at the company's specific pH value, each protein has a different charge amount and characteristics, so it can be achieved by studying ion-exchange chromatography technology Time separation. Like anion exchange chromatography, proteins with low negative charges elute first. Molecular sieves, also known as gel filtration, small proteins are inserted into the pores and have a long residence time, while large proteins cannot flow into the pores and are straight.
4. Protein purification equipment is used for protein purification, ultracentrifugation: it can be used to separate and obtain purified protein, and can also be directly used to determine the molecular weight of protein. Different impact proteins have different densities and morphological structures.