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  • Time of issue:2020-02-24 00:00:00
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a piece

Column efficiency test

Instrument: SCG-030

Column: Tricorn column, Source 15S

Test sample: 2M NaCl

Sample volume: 1.0% column volume

Detection: conductivity

 

 

a piece

Instrument: SCG-100

Sample: his-tag fusion protein

Column specifications: 1mL

Equilibrium: 0.05M Tris-HCl, 0.5M NaCl, pH 8.0

Elution: 0.05M Tris-HCl, 0.5M NaCl, 0.5M imidazole, pH 8.0

Sample flow rate: 0.5mL / min.

Experimental flow rate: 1mL / min.

 

 

a piece

Instrument: SCG-100

Column: Nano Q-15L, 1mL

Flow rate: 0.5mL / min

Method: Column washing 2CV, 100% A, gradient elution 100% A-100% B, 20CV

 

a piece

Instrument: SCG-100

Chromatography column: 21.2 * 250mm

Mobile phase: 6% ethanol

Flow rate: 10mL / min, collect samples

 

 

 

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Time of issue:2020-02-24 00:00:00

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