CASE
Applications
- Categories:应用案例
- Time of issue:2020-02-24 00:00:00
- Views:0
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Column efficiency test |
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Instrument: SCG-030 |
Column: Tricorn column, Source 15S |
Test sample: 2M NaCl |
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Sample volume: 1.0% column volume |
Detection: conductivity |
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Instrument: SCG-100 |
Sample: his-tag fusion protein |
Column specifications: 1mL |
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Equilibrium: 0.05M Tris-HCl, 0.5M NaCl, pH 8.0 |
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Elution: 0.05M Tris-HCl, 0.5M NaCl, 0.5M imidazole, pH 8.0 |
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Sample flow rate: 0.5mL / min. |
Experimental flow rate: 1mL / min. |
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Instrument: SCG-100 |
Column: Nano Q-15L, 1mL |
Flow rate: 0.5mL / min |
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Method: Column washing 2CV, 100% A, gradient elution 100% A-100% B, 20CV |
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Instrument: SCG-100 |
Chromatography column: 21.2 * 250mm |
Mobile phase: 6% ethanol |
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Flow rate: 10mL / min, collect samples |
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